OnsiteGene XDive® Ultra-Fast Real-Time PCR Instrument
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OnsiteGene’s XDive® 5-minute PCR is an ultra-fast real-time PCR analysis platform that integrates high-speed thermal cycling, ultra-fast PCR reaction reagents, and real-time fluorescence data acquisition workflows. It can complete a 40-cycle qPCR reaction in less than 5 minutes while maintaining the sensitivity and dynamic range of a conventional PCR instrument. XDive® can perform up to 16 ultra-fast reactions per experiment, providing moderate sample throughput and maximum working flexibility. It uses proprietary heating blocks, sensors, and algorithms to achieve ultra-fast ramp rates and superior temperature control accuracy. Its optical system consists of 4 fluorescence channels, which can be detected by multiplex real-time PCR analysis in minutes. XDive’s unique TaqMan™ chemistry has been optimized for ultra-fast operating conditions. XDive®reaction tubes accommodate reaction volumes on conventional PCR instruments, resulting in highly accurate and reproducible results for very ultra-fast fluorescence PCR analysis. The software structure of the system is simple and reasonable, and it is easy to operate.
XDive® as an integratable superfast qPCR module
OnsiteGene’s original XDive® 5-minute ultra-fast real-time PCR instrument can be easily integrated into new systems as an OEM module, significantly increasing productivity and flexibility, as well as reducing application costs. With OnsiteGene’s 5 minutes superfast magnetic bead nucleic acid extraction instrument (Xtractor™) and reagent, users can also develop new ultra-fast real-time PCR applications on the XDive® system with 16 sample throughput in as fast as 10 minutes from sample to answer, for point-of-care molecular testing, biosafety and biodefense testing, agricultural and animal health testing, food safety inspections, and environmental testing, etc.
XDive® completes 40 cycle real-time PCR testing in 4:54 minutes without sacrificing detection sensitivity
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4:54 minutes (40 cycles) of highly sensitive qPCR (CoVID plasmid). Can be shortened to 3 minutes.
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20μL reaction volume in each PCR tube.
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Unique superfast dried PCR and RT-PCR reagents.
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16 PCR tubes with 4 optical channels, capable of uniform temperature ramping and optical uniformity.
Key Product Highlights of XDive®
- 5 minutes to complete 40 cycles real-time PCR assay (20 μL reaction volume)
- 7 minutes to complete 40 cycles RT-qPCR detection (20 μL reaction volume)
- Patent granted in major countries around the world (United States, China, European Union, Japan, Australia, India, Singapore)
- IEC 61010 product safety certification and IEC 61326-2-6:2020 electromagnetic safety certification from TUV
- FDA emergency authorization for COVID-19 RT-qPCR Assay on XDive™
- FDA emergency authorization for SalivaDirect™ COVID-19 Assay on XDive™
- FDA emergency authorization for MonkeyPox COVID-19 Assay on XDive™
- ISO13485 production facility, including IVD equipment, consumables, IVD reagents and IVD infectious disease diagnostic kits.
- 5-minute superfast magnetic beads nucleic acid extraction instrument (Xtractor) and reagent (5 minutes for swab samples) to interface with XDive™ instrument.
- Superfast extraction-free reagents (less than 10 minutes to complete “sample-to-result” molecular detection of swab, blood, and fecal samples).
Specifications
| Technology | Real-time quantitative PCR with superfast thermal cycling |
|---|---|
| Heating speed outside PCR tube | 82.0°C per second |
| Cooling speed outside PCR tube | 67.0°C per second |
| Heating speed inside PCR tube | 28.5°C per second |
| Cooling speed inside PCR tube | 17.5°C per second |
| Optical channels | 4 channels, including FAM, VIC/HEX/SUN, ROX/Texas Red, CY5 |
| Reaction volume | 5 μL to 30 μL |
| PCR tubes per run | Rack: up to 16 tubes |
| Thermal cycling time with optical imaging | Min 3 minutes over 40 qPCR cycles with 20 μL reaction volume |
| Superfast PCR reagent | Liquid reagent, Dried reagent (PCR and RT-PCR), Direct-PCR reagent |
| Speed for RT-qPCR and qPCR | Min 7 minutes RT-qPCR for low copy RNA detection, Min 5 minutes qPCR for low copy DNA detection |
| Weight | 24.5 kg |
| Dimensions (W×D×H) | 342 × 450 × 357 mm |
Software
Harness the full potential of your research with RapidPCR data acquisition and analysis software, the perfect companion for the XDive® instrument’s capabilities. Designed with the user in mind, this intuitive software simplifies your journey from sample to insight. Input user details, fine-tune thermal cycling parameters, and set fluorescence imaging options with ease. The software’s robust analysis tools not only provide detailed reports but also offer the flexibility to export raw PCR data, ensuring your results can be further scrutinized or shared for collaborative studies. With XDive® instrument, your research is empowered by precision, efficiency, and collaboration.
Superfast Reagent
XDive® Reagent – Superfast PCR and RT-PCR reagent
1. Dried reagent
OnsiteGene’s superfast dried reagent is a high-performance PCR and RT-PCR reagent developed for XDive® applications. It performs highly sensitive detection under extreme high temperature ramping as compared to best liquid PCR reagents in the market. It is stable for 12 months under room temperature of 25°C degrees.
2. Liquid reagent
OnsiteGene’s superfast liquid reagent is a high-performance PCR and RT-PCR reagent developed for XDive® applications. It performs highly sensitive detection under extreme high temperature ramping significantly better as compared to best liquid PCR reagents in the market.
Superfast Test Kits
XDive® Test Kits (RUO)
- Superfast virus PCR detection kit for Customs
- Superfast food safety PCR kit
- Superfast respiratory pathogens PCR detection kit
- Superfast pathogens PCR detection kit for Import and Export quarantine
- CDC recommended pathogens PCR detection kit
- Parasitic and vector-borne diseases PCR detection kit
- Superfast Water pollution pathogens PCR detection kit
- CoVID test
- Respiratory panel
- Pneumonia panel (in pipeline)
- GI panel (in pipeline)
- STI panel
Quick Extraction-Free kit (respiratory swab sample)
For XDive® instrument, the kit can achieve “Sample-to-Result” respiratory pathogen testing in less than 10 minutes.
Quick Extraction-Free kit (blood sample) (in pipeline)
For XDive® instrument, the kit can achieve “Sample-to-Result” blood sample molecular testing in less than 10 minutes.
Quick Extraction-Free kit (fecal sample) (in pipeline)
For XDive® instrument, the kit can achieve “Sample-to-Result” fecal sample molecular testing in less than 10 minutes.
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| Technology | Real-time quantitative PCR with superfast thermal cycling |
|---|---|
| Heating speed outside PCR tube | 82.0°C per second |
| Cooling speed outside PCR tube | 67.0°C per second |
| Heating speed inside PCR tube | 28.5°C per second |
| Cooling speed inside PCR tube | 17.5°C per second |
| Optical channels | 4 channels, including FAM, VIC/HEX/SUN, ROX/Texas Red, CY5 |
| Reaction volume | 5 μL to 30 μL |
| PCR tubes per run | Rack: up to 16 tubes |
| Thermal cycling time with optical imaging | Min 3 minutes over 40 qPCR cycles with 20 μL reaction volume |
| Superfast PCR reagent | Liquid reagent, Dried reagent (PCR and RT-PCR), Direct-PCR reagent |
| Speed for RT-qPCR and qPCR | Min 7 minutes RT-qPCR for low copy RNA detection, Min 5 minutes qPCR for low copy DNA detection |
| Weight | 24.5 kg |
| Dimensions (W×D×H) | 342 × 450 × 357 mm |
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Price range: $5,699.00 through $6,495.00
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