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Techne Quantica Real Time PCR Thermal Cycler

  • 96-well low profile microplate format with heat sealed optical film
  • Temperature controlled heated lid between 10°C above ambient and 110°C
  • Ramp rates up to 2.6°C/sec
  • Temperature range 4°C – 99°C
  • Robot friendly CD-type block loading mechanism
  • Block uniformity of better than ±0.25°C
  • Gradient function available as an option (maximum gradient 30°C)
Product Description

The Techne Quantica® Real-Time Nucleic Acid Detection Quantifiable PCR Thermal Cycler System has been designed with the advantage of having an open architecture and chemistry format that allows the end user full flexibility of the methods and research they wish to pursue.

 Consider buying recertified? Check out the Opticon 2 Real-Time Cycle

The Techne Quantica® provides at least comparable sensitivity and reproducibility with greater flexibility than many competitive instruments currently on the market whilst using a standard 96 well format.

Techne Quantica® Quantifable Realtime PCR Thermal Cycler

Multiplex capability... chemistry versatility... Choose the Quantica Real-time Nucleic Acid Detection PCR Thermal Cycler from Techne

The real-time nucleic acid detection system from Techne has been designed with the advantage of having an open architecture and chemistry format that allows the end user full exibility of the methods and research they wish to pursue

Features of the Teche Quantica Real-time Acid Detection PCR Thermal Cycler

Solid state white light source and PMT (photomultiplier tube) detector gives an impressive excitation range of 470nm to 650nm and a detection range of 500nm to 710nm

Multiplex - multiple wavelengths are detectable per sample using up to four paired excitation and emission  lters housed in individual cartridge systems

Wide dynamic range - up to at least 9 orders of magnitude from starting copy number and high sensitivity detecting down to 1nM  uorescein and single copy templates depending upon the assay

Techne Quantica Heated Lid

The flexible optical heated lid system is designed to minimize loss of sample and prevent sample condensation. Temperature controlled it allows further method optimization where pre-incubation stages may be required (e.g. in reverse transcription techniques) or where changes in the heated lid characteristics are needed during the different stages of a program run.

  • 96-well low profile microplate format with heat sealed optical film
  • Temperature controlled heated lid between 10°C above ambient and 110°C
  • Ramp rates up to 2.6°C/sec
  • Temperature range 4°C – 99°C
  • Robot friendly CD-type block loading mechanism
  • Block uniformity of better than ±0.25°C
  • Gradient function available as an option (maximum gradient 30°C)

Quansoft® software and reporting

Accompanying Quantica® is our unique intuitive software Quansoft®. By employing a series of user-friendly windows Quansoft® enables any real-time experiment to be created with ease.

Comprehensive completely user customizable report formats can be selected from quantification dissociation curve plus/minus scoring and allelic discrimination analyses. Fully user-customizable report formats can be printed or exported to Adobe PDF while raw data formats can be exported to MS Word PowerPoint and Excel.

Quansoft® Software
Techne Quantica Realtime PCR Thermal Cycler
Refer to number for the corresponding screenshots

1. Quansoft Home Page
By employing a series of user friendly windows Quansoft enables any real-time experiment to be created with ease.

2. Experiment Editor
Using combinations of plate layout program and analysis parameters this unique Quansoft feature allows the user even easier management of experiment protocols.

3. Plate Layout Editor
Within a matter of seconds a 96 well microplate can be left blank or assigned with unknowns controls standards user defined samples or test samples. All are colour coded for ease of identification.

4. Program Editor
Individual cycles and steps or a ramp read are added quickly to build up and display the thermal program and read points.

5. Analysis Wizards
Select the analysis wizard of your choice to take you quickly through the setup of the analysis type and its associated parameters for your specific experiment either pre or post run.

Choose from:
  • None
  • Baseline corrected
  • Quantification
  • Dissociation curves
  • Plus minus scoring
  • Allelic discrimination
  • Multi-read
 
 The Optical Path


6. Results Editor
View and easily modify the results parameters of your experiment post run using Quansoft's multiple document interface (MDI).

Export results tables visually customized graphs or even your raw fluorescence data to most commonly used software packages.

7. Quantica Reports
Comprehensive completely user customizable report format can be selected for all the available analysis types.

Specifications for the Techne Quantica® Real-Time Nucleic Acid Detection Quantifable Thermal Cycler System

ParameterQuantica®
Block format 96x0.2ml
Block specification 8 x Peltier block employing quad-circuit technology to enhance performance
Block uniformity at 50°:C Better than ±:0.3°C
Maximum ramp rate Up to 2.2°C
Temperature range 4 to 98°C
Sample volume 15 to 50ul
Heated lid Adjustable between 100 and 115°C in 1°C increments or off
Maximum sample evaporation Less than 15 volume loss from any well using the plate sealing recommended by Techne
PC connectivity USB OS Win XP SP3 or higher and Win 7 (one unit per PC)
Plate format Low profile 96-well plate (skirted or non-skirted)
Excitation source Solid state white light source
Detector Photon counting photomultiplier tube
Multiplex dye detection Up to 4 dyes per reaction tube
User selec ted filters Maximum of 4 paired excitationemission filter cartridge systems suitable wth currently used dyes*
Fluorescence excitation range 470-650nm (standard filters are centered at 485 530 580 and 640nm)
Fluorescence detection range 500-710nm (standard filters are centered at 520 560 615 and 685nm)
Dynamic range At least 9 orders of magnitude of target DNA concentration
Sensitivity (detection threshold) 1nM fluorescein in a 20ul sample
Resolution 1.5 fold difference in target DNA concentration
Weight 55 lbs
Dimensions WxHxD in. 16 x 20 x 21
Input power 715W
Power supply 100-230V 5060Hz



Parameters of Product: Specifications
Ports USB
User Interface PC Compatible
Well Size @ 0.2ml Tubes 96 Well
Manufacturer Techne

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