BD FACSCalibur Flow Cytometer
- Fixed optical assembly
- Optical coupling
- Background rejection
- Forward scatter detector and filter
- Side scatter detector
- Fluorescence detectors and filters
- Forward and side scatter sensitivity
- Forward and side scatter resolution
The BD FACSCalibur Flow Cytometer is an innovative platform used for both cell analysis and cell sorting in a single benchtop unit. The system is designed with a multicolor application support which allows accommodation of a wide range of laboratory and clinical applications.
Engineered to improve laboratory productivity, the BD FACSCalibur system offers an easy-to-use software, multisample loading options, and intuitive instrument and fluidics control. The BD FACSCalibur system is fully modular, thus allowing easy upgrade to satisfy specific application needs.
Other added functions of the BD FACSCalibur include optical coupling, background rejection, clear signal detection, and red-diode (635-nm) laser signal rejection, among others.
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BD FACSCalibur Flow Cytometer Specifications
|Laser||488-nm, air-cooled Argon ion-laser, 15 milliwatt, life expectancy > 5,000 hours;
635-nm, air-cooled red diode laser (optional)
|Fluorescence Sensitivity||Estimated detection limit is 200 FITC equivalent molecules per particle|
|Fluorescence Resolution||Coefficient of variation in FL2-Area of|
|Forward and Side Scatter Sensitivity||Sensitivity enables the separation of fixed platelets from noise|
|Forward and Side Scatter Resolution||Scatter performance is optimized for resolving lymphocytes, monocytes and granulocytes|
|Rate||Up to 300 cells per second|
|Modes||Single Cell, Exclusion, and Recovery|