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Historically Techne has provided easily interchangeable thermal blocks and the Techne TC-PLUS (TC-5000 Pluse PCR Thermal Cycler) units are no exception. The three block options, 96 x 0.2ml, 60 x 0.5ml and 384-well plate blocks all have a gradient option, thus enabling easy optimisation of PCR conditions such as Mg2+ and primer concentrations and annealing temperatures.
To assist with PCR assay design a Tm calculator which uses the Nearest Neighbour algorithm is included, and combined with the gradient every experiment is guaranteed to be optimised first time, every time. The new block design incorporates the very latest in Peltier design and provides one of the fastest ramp rates available at 5ºC/sec. One new feature of the thermal block is the TERSG system, Thermal Energy Recovery System (patent pending) which harnesses the released heat during the cooling phase for use in the next heating phase, thus saving energy and reducing operational costs – a must in today's energy conscious world.
The TC-PLUS (Techne TC-5000+ Thermal Cycler) is easy to program with a large, 5.7" VGA colour touchscreen and graphical displays. At least 1000 programs can be stored on the TC-PLUS, so there is more than enough capacity for everyone in the lab.
An easy to access USB port on the front of the cycler makes it quick and easy to transfer protocols between both cyclers and laboratories.
The Techne TC-PLUS and Satellite units are designed with a front-loading sample drawer to be space-saving, having the unique ability to stack.
Bench space is at a premium in the modern laboratory so a TC-PLUS (Techne TC-5000+ Thermal Cycler) or a Satellite can be stacked onto another Satellite, with a 4-block system only requiring 0.21m2.
Each TC-PLUS (Techne TC-5000+ Thermal Cycler) can directly control up to 3 Satellites or 9 via a USB hub. Additionally the Satellites can also be controlled via a PC using the software supplied with every unit.
Prevention of sample evaporation, particularly with low volume PCR reactions is critical for achieving the right results every time and vital for preventing false negatives.
The unique drawer mechanism (patent pending) utilises the energy of the drawer being pushed closed to lower the heated lid into the correct position.
It accommodates all consumable types from 0.5ml microtubes to 384- well plates, sealed with either heat or adhesive seals. For applications requiring lower experimental temperatures than standard PCR the heated lid can be set between 35 and 115ºC.
Airflow during the heating and cooling phases is vital to the temperature control of the samples, so for improved performance the venting and airflow is now front to back on the TC-PLUS (Techne PCR TC-5000+ Thermal Cycler). This also enables side-by-side positioning.