The Techne Quantica® – Real-Time Nucleic Acid Detection Quantifiable PCR Thermal Cycler System has been designed with the advantage of having an open architecture and chemistry format that allows the end user full flexibility of the methods and research they wish to pursue.

The Techne Quantica® provides at least comparable sensitivity and reproducibility with greater flexibility than many competitive instruments currently on the market whilst using a standard 96 well format.

Techne Quantica® Quantifable Realtime PCR Thermal Cycler

Multiplex capability... chemistry versatility... Choose the Quantica Real-time Nucleic Acid Detection PCR Thermal Cycler from Techne

The real-time nucleic acid detection system from Techne has been designed with the advantage of having an open architecture and chemistry format that allows the end user full exibility of the methods and research they wish to pursue

Features of the Teche Quantica Real-time Acid Detection PCR Thermal Cycler

Solid state white light source and PMT (photomultiplier tube) detector gives an impressive excitation range of 470nm to 650nm and a detection range of 500nm to 710nm

Multiplex - multiple wavelengths are detectable per sample using up to four paired excitation and emission  lters housed in individual cartridge systems

Wide dynamic range - up to at least 9 orders of magnitude from starting copy number and high sensitivity detecting down to 1nM  uorescein and single copy templates depending upon the assay

Techne Quantica Heated Lid

The flexible optical heated lid system is designed to minimize loss of sample and prevent sample condensation. Temperature controlled it allows further method optimization where pre-incubation stages may be required (e.g. in reverse transcription techniques) or where changes in the heated lid characteristics are needed during the different stages of a program run.

• 96-well low profile microplate format with heat sealed optical film
• Temperature controlled heated lid between 10°C above ambient and 110°C
• Ramp rates up to 2.6°C/sec
• Temperature range 4°C – 99°C
• Robot friendly CD-type block loading mechanism
• Block uniformity of better than ±0.25°C
• Gradient function available as an option (maximum gradient 30°C)

Quansoft® software and reporting

Accompanying Quantica® is our unique intuitive software Quansoft®. By employing a series of user-friendly windows Quansoft® enables any real-time experiment to be created with ease.

Comprehensive completely user customizable report formats can be selected from quantification dissociation curve plus/minus scoring and allelic discrimination analyses. Fully user-customizable report formats can be printed or exported to Adobe PDF while raw data formats can be exported to MS Word PowerPoint and Excel.

Quansoft® Software Refer to number for the corresponding screenshots

1. Quansoft Home Page By employing a series of user friendly windows Quansoft enables any real-time experiment to be created with ease. 2. Experiment Editor Using combinations of plate layout program and analysis parameters this unique Quansoft feature allows the user even easier management of experiment protocols. 3. Plate Layout Editor Within a matter of seconds a 96 well microplate can be left blank or assigned with unknowns controls standards user defined samples or test samples. All are colour coded for ease of identification.

4. Program Editor Individual cycles and steps or a ramp read are added quickly to build up and display the thermal program and read points.

5. Analysis Wizards Select the analysis wizard of your choice to take you quickly through the setup of the analysis type and its associated parameters for your specific experiment either pre or post run.

Choose from: None Baseline corrected Quantification Dissociation curves Plus minus scoring Allelic discrimination Multi-read

The Optical Path

6. Results Editor
View and easily modify the results parameters of your experiment post run using Quansoft's multiple document interface (MDI).

Export results tables visually customized graphs or even your raw fluorescence data to most commonly used software packages.

7. Quantica Reports
Comprehensive completely user customizable report format can be selected for all the available analysis types.

Specifications for the Techne Quantica® Real-Time Nucleic Acid Detection Quantifable Thermal Cycler System