Molecular Devices SpectraMax 340PC384 Absorbance Microplate Reader

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Complete Product Description

The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Analysis Software.

The SpectraMax 340PC384 Absorbance Microplate Reader Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the visible wavelength range, with the only temperature-independent method of pathlength correction.

The patented multichannel optical design of the SpectraMax® 340PC384 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans.

The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays.

Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax 340PC384 Microplate Reader transforms each well in a microplate to a fixed optical path length cuvette. This temperature-independent normalization corrects for varying well volumes and can eliminate standard curves by allowing the calculation of concentrations directly from the absorbance with a known extinction coefficient, as exemplified with nucleic acid and protein quantification.

The SpectraMax 340PC384 Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Additionally, the SpectraMax 340PC384 Microplate Reader can be seamlessly integrated with the StakMax® Microplate Handling System through the SoftMax Pro Software.

Accurate Quantitation
No Interference Filters
Fluid Measurements

The optical design of the SpectraMax 340PC384 Reader truly mimics a dual-beam spectrophotometer. Each well has its own sample beam and reference detector. The eight-channel system, consisting of eight sample beams and eight reference beams, delivers both superior precision and speed-ofreading across the microplate.

The SpectraMax 340PC384 system has a monochromator instead of interference filters. Simply select the absorbance maximum of a sample and let the monochromator tune to that exact wavelength. Or scan up to 384 wells at any time to determine the best assay conditions. The wavelength and scanning ranges are from 340– 850 nm in 1 nm increments. This is equivalent to having 511 built-in filters, so the correct wavelength required is always available.

The patented† PathCheck® Sensor measures the depth of the liquid in each microplate well. This information can be used to normalize absorbance values to a 1 cm pathlength so they will agree with measurements made in a standard 1 cm cuvette. This feature can also be used to calculate concentrations without a standard curve and expand the dynamic range to 6+ OD. The PathCheck Sensor can accurately measure liquid volumes down to 5 μL to test the accuracy and precision of liquid handling devices.

Analysis Software
Optical Performance
Robot Integration

SoftMax Pro Microplate Data Analysis Software provides flexibility in experimental design, setup, analysis and reporting, providing the opportunity to customize assays exactly to user specifications. Users can password-protect data, choose from nine different curve-fitting routines and use default data reduction, or set up custom formulas for analysis. Data can be analyzed and combined from different plates. (See Figures 1, 2 and 3.) FDA 21 CFR Part 11 compliance tools are also available.

The optional SpectraTest® ABS1 Validation Package provides a NIST-traceable solution for validating the SpectraMax 340PC384 system. The following measurements are automatically made by SoftMax Pro Software: baseline noise, dark noise, optical alignment, wavelength accuracy, photometric linearity and wavelength precision.

The SpectraMax 340PC384 Reader can be integrated with Molecular Devices' StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The "out-of-the-box" automation solution saves up-front integration time and resources.

Applications The SpectraMax 340PC384 System can handle a wide variety of applications including:

ELISA/EIA
Cytoproliferation/cytotoxicity
Kinetic ELISAs/enzyme assays
ß-galactosidase reporter gene
Colorimetric protein
NAD(P)H-based assays

Photometric Performance
Wavelength Range	340-850 nm
Wavelength Selection	Monochromator, turnable in 1 nm increments
Wavelength Bandwidth	2 nm
Wavelength Accuracy	+/- 1 nm
Wavelength Repeatability	+/- 0.2 nm
Photometric Range	0 to 4.000 OD
Photometric Linearity (405 nm)	0 to 3.000 OD
Photometic Accuracy	< +/- 0.0006 OD +/- 1.0%, 0-2 OD
Photometirc Precision	< +/- 0.0003 OD +/- 1.0%, 0-2 OD
PathCheck Sensor Measurement Error	< 5% compared to 1 cm cuvette
Microplate Single Wavelength
Endpoint	96 wells in 9 secs 384 wells in 29 secs
Minimum Kinetic Interval	96 wells in 9 secs 384 wells in 29 secs
Temperature Regulation
Temperature Range	Amb 4-45 C
General Specifications
Dimensions (in.)	8.6(H) x 22.8 (W) x 15 (D)
Dimensions (cm.)	22(H) x 58(W) x 38(D)
Weight	30 Ilbs. (13.6kg)
Power Consumption	<250 watts
Powersource	100-240 VAC, 4A 50/60Hz
Robot Compatible	YES