
New Brunswick BioFlo 3000 Laboratory Fermenter is the gold standard laboratory scale fermenter. It is an advanced bio processing system designed to maximize yields in both fermentation and cell culture. Ideally suited to meet the changing needs of the research laboratory- this versatile fermentor/bio reactor is capable of producing high yields of biomass or secreted products from virtually any microbial- animal- insect and plant cell line. The stirred-tank system comes complete with everything needed for out-of-the-box fermentor start-up- and is offered with a variety of options for dedicated cell culture applications.
BioFlo 3000 is equipped with a thick-walled glass tube fermentor vessel that provides superior resistance to rough handling and repeated autoclaving. This configuration allows for significant reduction in autoclaving time compared to glass-jacketed vessels. For precise temperature control the stainless-steel base has a dished bottom that is jacketed for the circulation of warm or cool water. The BioFlo 3000 provides a closed-loop temperature control system without the need for external water baths or circulators. The vessels are interchangeable and have working volumes of 1.25- 2.5- 5.0 and 10 liters. All seals are EPDM- silicone or Teflon.
Easy handling- set-up- and installation are achieved by a detachable motor that readily couples to the agitator without tools- convenient quick-connects for jacket water circulation- and alignment pins that secure the vessel to the console without tools.
This Laboratory fermenter has an array of beneficial features all of which are outlined below.
Built-in Gas-Mixer Optimizes Dissolved Oxygen Levels For High-Density Culture. Two-Gas Mixing in Fermentation provides automatic or manual blending of supplemental oxygen and air. Create air- oxygen- and agitation control cascades that respond to oxygen-demanding microbes- while limiting foam and shear. Four-Gas Mixing in Cell Culture- blends air- oxygen- carbon dioxide- and nitrogen for precise and gentle automatic control of both pH and dissolved oxygen
350 mM/L/Hr Oxygen Transfer Rate obtained through powerful fermentation agitation motors and 6-bladed Rushton impellers
Choice of 12 Interchangeable Vessels. Fermentation vessels offered with or without side ports- in four sizes from 1.6 through 14 liters. Glass-jacketed cell culture vessels- 2.2 through 14 liters total volume
Stainless-Steel Heat Exchanger forms the rounded bottom of fermentation vessel assemblies. Allows the shortest autoclave time- with no impeding double-insulating glass to slow sterilization. Large-area high-conductivity steel surface provides superior temperature uniformity and the most efficient heat-transfer characteristics. The best choice for fermentation- especially for high-density cultures and for temperature induction
Optional Low Side Entry Ports (available in fermentor vessels only) - two 19 mm ports in the stainless-steel heat-exchanger support additional probes- which enter the vessel below the impeller. 12 mm adapter available
Other features include:
- Vessel Side Ports support chemostat operation
- Integrated Vessel- Console- Controller and Pumps conserve bench space and simplify connections
- Choice of Thermal Mass Flow Meter or Thermal Mass Flow Controller for accurate air flow monitoring and control
- Tunable P-I Values Optimize DO Control
- Five Assignable- Peristaltic Pumps built in; with control included for optional sixth pump
- Stainless-Steel Exhaust Condenser minimizes media evaporation
- Stainless-Steel Ring Sparger provides uniform distribution of bubbles to impeller blades
- Battery Back-Up retains settings in the event of a power failure
| Agitation | |
| Drive | Permanent magnet top-drive motor with high torque. Readily removable without tools. |
| Range | 50 to 1000 rpm5 |
| Sensor | Photo-optical encoder disk with a resolution of 1000 pulses/revolution |
| Control | Microprocessor-based with PID |
| Impellers | Six-bladed Rushton impellers standard. Other impellers optional |
| Indication | Digital display in 1 rpm increments |
| Temperature | |
| Range | From 5 |