Biotech Clinical Microplate Reader Molecular Devices Gemini EM Fluorescence Microplate Reader
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Molecular Devices Gemini EM Fluorescence Microplate Reader
- Top and bottom reading
- No need for filters
- Higher Sensitivity
- Validation Tools Available
- Robotics compatible
- Application Versatility
Complete Product Description
The Gemini EM Fluorescence Microplate Reader enables top and bottom reading of microplates over a greater range of fluorescence intensities—all without the use of filters.
The Gemini™ EM Fluorescence Microplate Reader exemplifies flexibility for fluorescence assays. Reading 6 to 384-well microplates, the optical design of the instrument can be switched from top to bottom read modes for improved sensitivity to solutions and cell-based assays. Dual monochromators for variable wavelength selection between 250 nm and 850 nm eliminate the need for searching out the right pair of excitation and emission filters and wavelength scanning across a range of wavelengths in increments as small as 1 nm can be used to optimize assay parameters. Up to 4 wavelength pairs can be read for endpoint and kinetic measurements, and the Gemini EM Microplate Reader offers well scanning to report a fluorescent measurement from a single point in the center of a microplate well to multiple points across a tissue culture well.
Unlike most fluorescence readers that may saturate out with signal intensities over 3 orders of magnitude, the patented AutoPMT Optimization System of the Gemini EM Microplate Reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays so that low and high signals can be captured from the same plate. This calibration against an internal standard provides an additional benefit in being able to confidently compare relative fluorescence units (RFUs) of individual samples across plates and readers.
The Gemini EM Microplate Reader is supplied with SoftMax® Pro Data Acquisition & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Additionally, the Gemini EM Microplate Reader can be seamlessly integrated with the StakMax® Microplate Handling System through the SoftMax Pro Software.
Key Features
- Read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes.
- Eliminate the need for identifying, purchasing, and changing out filters for wavelength optimization with dual monochromators for excitation and emission wavelength selection.
- Measure fluorescence from solutions or cell-based assays with the best optical sensitivity by switching between top and bottom fluorescence reading.
- Avoid losing high signals to detector saturation and photobleaching after repeated reads to find the correct reader settings with AutoPMT System electronics.
- Compare relative fluorescence units (RFUs) between samples, like with no other fluorometer, allowed by a unique calibration against an internal standard.
- Monitoring of temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C.
- Thorough mixing of samples in microplate wells by user-programmable Automix feature.
- Gentle movement of cell or other aggregates in the microplate with a slow carriage speed option.
- Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. Molecular Devices provides complete solutions with:
- SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes.
- IQ/OQ/PQ Instrument Qualification documentation
- SoftMax Pro Software validation package
- Software tools for FDA 21 CFR Part 11 compliance
Applications
and Cytotoxicity | ||
SoftMax Pro
SoftMax Pro Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software, offers:
- The most extensive library of supported application protocols to get you started faster
- Optional tools for regulated labs with SoftMax Pro GxP Compliance Software
- Advanced calculations including non-linear fitting metrics for in-depth analysis
- The only Mac- and Windows-native application support for seamless exchange of data files between operating systems
- Proven integrations with leading automation and LIMS platforms for partnered solutions
| General Specifications | |
| Dimensions (in/cm): | 8.6 (h) x 22.8 (w) x 15 (d) 22 (h) x 58 (w) x 38 (d) |
| Weight | 35 lb (16 kg) |
| Power source: | 100-240 VAC, 50/60 Hz |
| Performance Specifications | |
| Wavelength Range - Excitation: | 250-850 nm |
| Wavelength Range - Emission: | 360-850 nm |
| Wavelength Selection: | Monochromator, tunable 1.0 nm increments |
| Wavelength Bandwidth (Excitation and Emission): | 9 nm |
| Wavelength Accuracy: | < ± 2.0 nm |
| Top Read Sensitivity: | 3.0 fmol/well FITC 200 µl in 96 wells |
| Light Source: | Xenon flash lamp |
| Microplate Read Time | |
| Normal Mode | 96 wells. . . . 15 seconds 384 wells. . . . 45 seconds Dynamic Range: 6 orders of magnitude Temperature Range: Ambient + 4 °C up to 45 °C Temperature Uniformity (Microplate): ± 1 °C @ 37 °C |
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