HP 1090 Series HPLC system
The HP 1090 Series HPLC system introduced in the early 80's is regarded to be an outstanding instrument in terms of performance and reliability. It was developed with the objective to create an HPLC system with optimized pump performance at 50–5000 μl/min flow rates and with a new type of detector that could acquire spectral data online. The result was a high performance solvent delivery system (DR5) and a new UV detector based on diode array detection technology (DAD)
HPLC 1090 system was introduced in 1983 and when it comes to performance and reliability it is considered to be an outstanding instrument before the introduction of the modular designed HPLC.
HP 1090 HPLC’s pump is being considered as the best pump available in the market today. The delay volume of HP 1090 is comparable to that of the Agilent 1100 series binary pump.
The HP 1090 HPLC uses helium for solvent degassing. The good thing about using helium degassing is that there is no need for additional internal volume to be added and changing solvent in one channel only takes a few minutes.
HP 1090 injector has a glass syringe for metering devices. This device can be used at low pressures. If the solvent needs to be change or if an air bubble needs to be removed it is advisable to use a special wash cycle in order to flush the syringe.
The HP 1090 Series performs a temperature control for its column compartment. This is being done by circulating the heated air inside the compartment and by preheating the eluent when it enters the compartment. Sometimes external cooling devices are needed to set the temperature right.
The goal of HP 1090 is to make sure the temperature in the column is the same on what it is being set. This was put into action by increasing the set temperature by previously estimated factor which was reliant on the flow rate and the set temperature.
It is legendary for introducing the first self metering pump which enables such superb precision.
This High-Performance Liquid Chromatograph is complete and consists of the following integrated modules:
a) Solvent delivery system
c) Column compartment
e) Waste reservoir
f) Optional HPLC compatible data analysis software
The HP 1090 chromatograph with a DR5 solvent delivery system which can have one two or three solvent channels active. Each solvent channel comprises a solvent reservoir and a dual-syringe metering pump with rotary valve. The channels are mixed in the low pressure compliance before being pumped at high pressure via a damping unit through the injector and onto the column. This system allows you to run in isocratic mode (only one solvent) binary (50:50 75:25...) or ternary (40:40:20 35:35:30...) and also it can be programmed to run with a gradient elution. A HP 1090 chromatograph is also equipped with a helium inlet line for solvent degassing which removes other gases dissolved in the solvents
A HP 1090 Chromatograph has both a manual injector and an autoinjector. The first one allows you to do only a single injection and the second can be programmed to do up 99 injections in a sequence. The sample injection valve is a six-port rotary valve designed for high performance liquid chromatography. When an injection is started an air actuator rotates the valve: solvent goes directly to the column; and the injector needle is connected to the syringe. The air pressure lifts the needle and the vial is moved into position beneath the needle. Then the needle is lowered to the vial. The sample is drawn up into a sample loop by the syringe metered by a stepper motor. The needle is raised a second time to allow the vial to move away. Then the needle is lowered a second time and the air actuator reverses the valve reconnecting the sample loop to the solvent flow. All the sample is flushed out of the injector reaching the column as an undiluted plug. Finally the syringe stepper-motor moves the syringe plunger to the end of the syringe sending the remaining solvent to the waste.