|
|
Bio Chromatography
FPLC- Fast Protein Liquid Chromatography System
GE Pharmacia FPLC
(GEPHFPLC)
The GE Pharmacia fast protein liquid chromatography (FPLC) system is used for methods development and the purification of large quantities of various bimolecular (i.e.- protein and DNA). The system includes two pumps capable of continuous flow to the purification columns (vary by user) - a peristaltic pump may be used for column equilibration- a UV monitor to detect bimolecular on the column- a mixer to produce elution gradients- motor valves may be used to assist with sample injection- column selection or flow reversal- and a controller with an integration function for computerized operation of various programs directing flow to the pumps and controlling the motor valves. A chart recorder is the typical mode of run documentation.
...a classic that stands the test of time and will supersede the trendiness of the age...
| Flow Range | |
| Isocratic mode | 0.05?20 ml/min in steps of 10 ul/min |
| Gradient mode | 0.1?20 ml/min in steps of 10 ?l/min |
| System | |
| Pressure Range | 0?5 MPa (50 bar- 725 psi) |
| Pressure pulsation | Max. 6 percent (dP/P) during pump stroke |
| Viscosity | |
| Less than 10 ml/min | Max. 10cP |
| More Than 10 ml/min | Max. 5cP |
| Dimensions | |
| H x W x H inch | 14.6 x 18.9 x 18.5 |
| Weight | 110 Ilbs |