Bio Chromatography
FPLC- Fast Protein Liquid Chromatography
GE Pharmacia AKTA Purifier
(WS-AKTA10)
The GE Pharmacia AKTA Purifier (10 or 100) is the new "classic" (remember the legacy FPLC) used for the rapid purification of proteins at low to medium pressures. high performance purification and characterization. Using state-of-the-art computer-controlled pumps, UV and conductance monitors, as well as fraction collector, the AKTA FPLC will greatly increase your protein purification efficiencies - indispensable when working with labile biomolecules and enzymes.
The GE Pharmacia AKTA Purifier is a high end liquid chromatography system designed for fast and reliable separations of peptides and nucleic acids. AKTApurifier runs all techniques, from ug to mg scale at flow rates 0.001 ml/min ? 10 ml/min and pressures 0?25 MPa. With operational simplicity and a wide range of pre-programmed application protocols and method templates, AKTApurifier incorporates many features to minimize preparation and run times and maximize reliability and reproducibility of results. AKTApurifier is essential to guarantee fast, reproducible runs everyday, as shown.
AKTApurifier systems are part of AKTAdesign, a range of liquid chromatography systems for academic and industrial research laboratories. AKTAdesign systems are controlled by UNICORN , a control, evaluation and reporting platform which also controls production systems at kg scale. Designed for Windows NT, UNICORN ensures quick, simple communication between systems and users and meets the stringent control and data handling procedures of modern laboratories. Optimized methods are transferred easily to production scale
AKTApurifier core systems, together with AKTAdesign automation kits and hardware, ensure that purification efforts are always focused on target proteins of interest. Purification can be performed at microgram, milligram or gram scale, depending on the core system selected. All this translates into improved versatility and tailor-made solutions for a specific application workflow of interest. Optional AKTAdesign automation kits and hardware allow functionality to be added on when application needs change.
There are four core AKTApurifier systems with essential functions for versatile FPLC purification. For purification of proteins at microgram and milligram scale, choose AKTApurifier 10 or UPC 10 core systems. Purification of larger, gram scale quantities of protein is easily achieved with AKTApurifier 100 or UPC 100 systems.
AKTApurifier 10 and 100 systems
AKTApurifier 10 and 100 core systems include Monitor UV-900 for UV-Vis detection of up to three wavelengths simultaneously. This allows visualization of more proteins when adequate detection at 280 nm cannot be achieved. Optional for AKTApurifier 10 and 100 core systems is Monitor pH/C-900, a combined monitor for accurate, online measurement of pH, conductivity, and temperature.
AKTApurifier systems are versatile liquid chromatography systems for protein purification by FPLC.
AKTA?, FPLC?, BioCAD, big, little, Fast, slow, beginner, advanced, I've got a method, I have no clue....which one to choose i don't know
OK, so which one should i buy?
AKTAPurifier 10 systems is an excellent choice if you are just 'starting out' or for those who routinely use columns that generate higher back pressures, AKTAPurifier 10 systems are available with a flow rate range up to 10 ml/min and pressures up to 25 MPa.
AKTAexplorer 100 systems is an excellent choice for those with somewhat unlimited budgets. The AKTAexplorer 100 is suitable for method development producing flow rates up to 100 ml/min and pressures up to 10 MPa. Equipped with air sensors, it becomes AKTAexplorer 100 Air, monitoring air infiltration at the sample and buffer inlets.
AKTAPilot systems is THE choice for those whose method is written and need to produce oodles and oodles (buckets and kilograms) of product. The 'Pilot' is capable of huge flow rates.
....by the way.....if you can't afford an AKTA, How about the same functionality is a smaller price point? Consider the BioCAD family....see below
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Did you know that GMI is the number one provider of guaranteed recertified BioCADs worldwide? !