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GE ÄKTA FPLC™ Fast Protein Liquid Chromatograph

  • The ÄKTAFPLCâ„¢ is built on Proven technology- Method wizard.
  • A comprehensive column library is availible for the ÄKTAFPLCâ„¢.
  • Automatic scouting for purification development and optimization.
  • The ÄKTAFPLCâ„¢ has short separation times using modern media.
  • Robust design is a key charactoristic of the ÄKTAFPLCâ„¢
  • Expect consistent reproducibility with high sensitivity with the ÄKTAFPLCâ„¢.
  • ÄKTAFPLCâ„¢ produces efficient fraction collection.
  • The ÄKTAFPLCâ„¢ can perform automatic evaluation and produce report procedures.
  • ÄKTAFPLCâ„¢ is expandable with automated functionality
  • Choice in ÄKTAFPLCâ„¢ system configuration options include: - Automatic column and buffer scouting. - Automatic multiple sample injection.
  • The ÄKTAFPLCâ„¢ can perform almost all chromatographic techniques.
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Complete Product Description

Overview of the GE ÄKTA FPLC™ Fast Protein Liquid Chromatography System

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GE ÄKTAFPLC™ has features that make it a fast easy protein purification system

  • Pre-programmed method templates
  • Comprehensive column library
  • Automatic scouting for purification development and optimization
  • Short separation times with modern media

GE ÄKTAFPLC™ offers reliability in every run!

  • Consistent reproducibility with high sensitivity
  • Efficient fraction collection
  • Automatic evaluation and report procedures
  • Easy, fast scale up

With the GE ÄKTAFPLC™ from GMI, you have a choice in system configuration including:

The GE ÄKTAFPLC™ offers flexibility

  • Automatic column and buffer scouting
  • Automatic multiple sample injection
  • All chromatographic techniques

With GMI and the services availible from AKTAdesign, you will have all the support you need for your new GE ÄKTAFPLC™ FPLC system

  • Flexible GMI service Agreements
  • AKTAdesign knowledge
  • AKTAdesign networks
  • AKTAdesign service

The GE ÄKTAFPLC™ Fast Protein Liquid Chromatography system (formerly Amersham Biosciences) is a new, bio-compatible, high performance liquid chromatography system for fast and easy purification of proteins. The unique design provides a compact, ready to use chromatography system with an expandable rack. The rack system gives built-in flexibility for the use of longer chromatographic columns, such as for gel filtration, changes in system configuration or increased automation. The GE ÄKTAFPLC™ is controlled by UNICORN, the control, evaluation and reporting platform for all AKTAdesign systems, ensuring quick and simple communication between systems and users.

GE AKTA FPLC

UNICORN control is designed for Windows NT and includes an option for network control of multiple systems.


GE AKTA FPLC with accessoriesGE ÄKTAFPLC™ is compatible with all solvents commonly used in protein purification, including the high salt concentrations used in hydrophobic interaction chromatography.

GE ÄKTAFPLC™ fast protein liquid chromatograph (FPLC) is the ideal FPLC system for your laboratory. Proteins of various sizes can be readily purified using different kinds of columns. The GE ÄKTAFPLC™ is a dual-pump system which is largely sufficient for most of our chromatography applications. FPLCs with four pumps are also available which provide a facility for running four different solutions simultaneously. The flow rate of the pump of the GE ÄKTAFPLC™ can get as low as 0.01ml per minute and can reach up to 10ml per minute. The pumps require a constant flow of 20% ethanol in water which serves as a lubricant. The GE ÄKTAFPLC™ system is provided with an online UV detector which can be set for a maximum of three wavelengths covering a range from the far UV region (214nm) to the visible range (700nm).

Injection loops of different volumes are available for the GE ÄKTAFPLC™ system. Loops of 10 – 150ml (superloops) and 100 – 150ml loops are also availible. A fraction collector that can gather up to 100 fractions either dropwise or at specified time intervals is provided with the GE ÄKTAFPLC™ system. The fraction collector can be automated or operated manually.

The software provided with the GE ÄKTAFPLC™ system is extremely easy to handle and supports a multi-user environment. Default methods for almost every column are available with the software provided. The GE ÄKTAFPLC™ can either be controlled manually or using programmed runs. A series of default programs for different kinds of proteins and conditions can easily be set up. This will help when re-purification of several batches need to be carried out. Variable pressure limits of different columns can quickly and easily be set. This option is extremely useful as the software automatically shuts down the pumps and pauses the run when the pressure exceeds the maximum cut-off. This can save the you expensive columns during your procedures! Screenshots of AKTAFPLC software

GE ÄKTA FPLC™ Fast Protein Liquid Chromatography system Specification


Parameter Value
Fast rate range 0.5-20 ml/min
Pressure Range 0-5 MPa (50 bar, 725 psi)
Wavelength Selection 254, 280, 313, 405, 436, 546 nm (with the Hg Lamp)
214 nm (with the Zn Lamp)
Conductivity Range 1μS/cm-999.9 mS/cm
(RPC- IEX- HIC gradients)
pH Range 0-14
Solvent Compatibility All solvents and buffers commonly used in chromatography of biomolecules, except 100% hexabe abd 100% tetahydrofuran (organic solvents are pottentially harmful to pH electrodes, usea dummy flow cell when running organic solvents)
Size 380 x 480 x 470 mm (W x D x H)
Weight 50 kg