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MJ Research PTC 100 Thermal
Cycler
The Classic, the one , the
only... !
Features &
Specifications:
- Hot Bonnet heated lid incorporates a thumbwheel to adjust
height and pressure. This feature assures tight sealing of
reaction vessels even microplates during oil-free thermal
cycling
- Peltier-effect heat pumps robustly designed for
repeated cycling deliver the full temperature range of 0°-100°C
electronically
- Speedy ramping rates of up to 1°C/sec in models that
hold 0.5ml tubes, 96-U-well or 192-well plates; up to 1.4°/sec in
models that hold 0.2ml tubes or V-well plates; up to 2.5°/sec in
models with the silver-gold block.
- Temperature homogeneity: ±0.4°C within 30 seconds of
arrival @ 60°C for all standard models.
- Memory stores approximately 320 programs, and
cycler comes with 14 preprogrammed protocols. Any sequence of
temperatures can be cycled up to 10,000 times; ramping rates less
than maximum may be specified.
- Extremely accurate incubations, with NIST-traceable
calibration. Any temperature—
including below-ambient temperatures—
may be held indefinitely. This feature allows refrigeration after
an unattended run.
- Parallel printer port
(optionally) allows the instrument to print
thermal profiles in real time and document programs in hard copy.
Additionally, an analog jack can output to a 0-5 volt chart
recorder.
- Power-failure protection will resume program after power
interruptions of up to twelve hours.
MJR PTC-200-The
MJ Research PTC-200 is a 96 well gradient thermocycler that
can be supplied with different 'alpha blocks' such
as a 96 well or a
dual 48-well block. The dual
48-well block allows two independent programs to be run at the same
time. The PTC-200 also uses the Hot Bonnetª to allow oil-free
operation. Precise Peltier-effect heat pumps --robustly designed for
repeated cycling-- deliver the full temperature range of 0-100C
electronically, in a compact instrument with a footprint that measures
24cm x 28cm x 27cm high. Speedy ramping rates of; up to 3.0¡/sec in
models that hold 0.2ml tubes or V-well plates; Unparalleled
temperature homogeneity: ±0.4C within 30 seconds of arrival @ 60C for
all standard models. Large memory & amiable software that allows
control over virtually every thermal parameter. Cycler has
80-character LCD, menu-driven software, and 200-program memory with
individually secured directories.
The MJ Tetrad with 4 alpha
blocks
4-bay high-throughput
thermal cycling
Up to 8 individual sample
blocks, which can be controlled independently or as a group
Up to 1536 samples can
be processed simultaneously
Internal power supply-no
separate "boxes" to accommodate
High-density color display
and on-board mousepad, with point-and-click navigation of software
Real-time monitoring
of cycling temperature
Extraordinary temperature uniformity
of ±0.4°C within 30 seconds of arrival at 90°C, well-to-well average
(±0.5°C for dual block Alphas).
Superb thermal accuracy
of blocks within ±0.3°C of NIST standard at 90°C, average across block
(±0.4°C for dual block Alphas).
Thermal range of
-5° to 105°C
Rapid ramp rates
of up to 3.0°C/sec
In stock now
$
Inquire at 763-712-8717 for your quotation
PTC200
This thermal cycler is a
wonderful product. Aside from being easy to use, those
equipped with the MJ research gradient block
extend capability. The gradient feature allows the temperature
to vary as much as 24 degrees from the left side of the block to the
right side. The means that you are able to have 12 different
temperatures on one block. This is useful for optimizing conditions
for new primer sets. All one has to do is create master mixes and
distribute among the twelve wells across the block. This is a huge
time saver and does the work of 12 pcr machines with just one
instrument.
This machine works well with many types of
tubes and does not squish tubes as does some other machines that we
have. This is most likely due to the deepness of the wells in the
block.
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Specifications for PTC 200 |
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Gradient capab. |
some with and some without |
| Thermal
Uniformity: |
±0.4°C
within 30 seconds of arrival at 90°C |
| Thermal
Accuracy: |
Average
temperature within ±0.3°C of programmed value at 90°C, NIST-traceable |
|
Temp range |
-5.00 to 105 |
|
Heating Cooling Rate |
3.00
C/sec |
| Reaction
blocks: |
can
contain 1
Alpha-Unit (11 different possibilities) |
PTC 100s in stock now
$
Inquire at 763-712-8717 for your quotation
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MJ Research
PTC 100 Thermal Cycler
MJ Research PTC 200 Thermal Cycler
MJ Research PTC 225 Thermal Cycler
MJ Research Tetrad Thermal Cycler
MJR PTC-100
The MJ Research PTC-100 is a 96 well thermocycler that also uses a
heated lid for oil-free operation plus the following features:
Improved Hot Bonnetª heated lid incorporates a thumbwheel to adjust
height and pressure. This feature assures tight sealing of reaction
vessels --even microplates-- during oil-free thermal cycling.
Precise Peltier-effect heat pumps --robustly designed for repeated
cycling-- deliver the full temperature range of 0-100C
electronically, in a compact instrument with a footprint that
measures 24cm x 28cm x 27cm high. Speedy ramping rates of; up to
1.4¡/sec in models that hold 0.2ml tubes or V-well plates;
Unparalleled temperature homogeneity: ±0.4C within 30 seconds of
arrival @ 60C for all standard models.
NEWS FLASH ! GMI carries brand new in the box thermal cyclers
with superior characteristics to the MJ PTC 200 line!
The FIRST with touch screen control !
ATC 201 96 well cycler
ATC 401 gradient thermal cycler
Call your sales rep for brochure and quotation
today
or by
completing the form on the
Ordering Page.
These brand new cyclers are real 'head turners' with full 2
year warranty and superior performance !
The last offering from MJ is the
PTC-200 DNA Engine. The sleek design and rugged chassis are
suggestive of an Italian sports car. Interchangeable sample blocks
and heat pump assemblies allow the user to change format easily
between runs while retaining NIST-traceable accuracy. The lab need
only buy the single control unit and supplement it with the desired
Alpha modules, such as dual, independently controlled blocks,
microplate format, or a slide adapter for up to 16 slides. Thermal
control is achieved by either block, in-sample probe, or calculated
methods. Heating rates up to 3°C/second reduce reaction times while
±0.4°C temperature homogeneity can be reached within 30 seconds at
90°C. The Hot Bonnet heated lid controls pressure and height to
accommodate various plasticwares. A Power Bonnet is available for
robotic systems. Additionally, a single cycler can be networked to
control up to 15 separate instruments. A PC can also control and
monitor cyclers remotely and export data to video screens or
databases in real time.
 For high-throughput applications or busy
labs, the PTC-225 or 'Tetrad' DNA Engine Tetrad may be the way to go. This
allows up to eight separate blocks to run synchronous or independent
reactions. A compact footprint is maintained by housing the power
supply separately outside the chassis, good for tight spaces or
robotic applications. All of the features of the PTC-200 are present
in this model as well.
The PTC-100 cyclers incorporate a Peltier
heating element into a more economical model that can be configured
to accommodate plates, slides, or tubes. An optional heated lid is
available as well as slide griddle adapters for amplifying up to
four slides at a time. A large memory that holds up to 320 programs
means that even crowded labs have ample programming space.
For small labs or those that want an
auxiliary cycler, the PTC-150 MiniCycler series may be just the
ticket. Incorporation of the Peltier-Joule heat pump increases
cycling speed with ramping rates up to 2.4°C/second. The temperature
may be programmed to allow refrigeration after completion of the
reaction. The compact, lightweight unit can be easily transported to
different areas of the lab. Power failure protection resumes cycling
after temporary power loss up to 12 hours.
Two models are available for in situ
work. The PTC-200/225 models mentioned above allow use of the Twin
Tower block that can hold up to 16x2 slides. The PTC-100-16MS is a
dedicated configuration of the PTC-100 series, able to hold up to 16
double- or single-thickness slides. The sample block is made from
oxygen-free copper milled from a single ingot and then plated in
nickel for durability. Memory for 200 programs, power-failure
protection, printer port, and a two-year warranty are standard.
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Thermal
range |
0° to 100°C |
|
Ramping
Speed |
60, 192: up to 1°/sec
96V: up to 1.4°/sec
96AgV: up to 2.5°/sec
16MS: up to 0.5°/sec |
|
Accuracy |
Average temp.
within ±0.5°C of NIST-traceable standard after 30 sec. @ 60°C |
|
Homogeneity |
±0.4°C within 30
sec of arrival @ 60°C |
|
Input
power |
100-240VAC 50/60Hz
350 watts maximum
2 x 4A 250V fuses |
|
Display |
32-character LCD,
3 LEDs, audible alert |
|
Memory |
320 typical
programs |
|
Port |
Parallel printer,
prints report of thermal profiles of block or probe |
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Weight |
approx. 7.5 kg |
|
Size |
24x28x27cm high |
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Operating
environment |
4° - 32°C ambient
10% - 90% humidity |
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Block
format |
96 x 0.2ml tubes or V-bottom 96-well
plate, other accessories are available |
You can reserve/order this instrument
by calling 763-712-8717 or by
completing the form on the
Ordering Page. |
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Other Thermal Cyclers:
NOTE: GMI carries brand new in the box thermal cyclers with superior
characteristics to the MJ PTC 200 line!
MJ Research PTC
100 96 well thermal cycler
MJ Research PTC
200 96 well thermal cycler
MJ Research
Tetrad thermal cycler
Perkin
Elmer PE 9600
Perkin
Elmer PE model PE 9700
ABI 7700 Real Time PCR
This figure shows the amplification of samples from a dilution series
done with an ABI 7700 real time PCR
Tetrad
thermalcyclers
contains an onboard Pentium®-class computer that delivers an
advanced graphical interface-ideal for managing production-grade
thermal cycling. For those labs that value quick training, the
creation and editing of protocols can be accomplished rapidly and
easily via the mouse-directed -point-and-click- navigation.
Real-time displays of block, sample and lid temperatures provide
quality assurance that the thermal cycler is performing as directed.
Superior software features, such as error tracking logs, run
tracking files, problem resolution trees and file management tools,
offer invaluable information for troubleshooting during high
throughput runs.
This device is
a large capacity thermal cycler specifically designed for
high-throughput amplifications.
The DNA Engine
Tetrad consists of four sample-block/heat-pump modules suitable for
amplifications in either 96- or 384-well format. The temperature
control is provided by Peltier-Joule heat pumps which samples
thermal profiles every 50 milliseconds. The temperature of the
heatings blocks can be easily modulated at a rate of 3.0°C/sec
within a range from –5.0°C to 105°C. We perform our amplifications
in 384-well plates, but as mentioned above, it is also possible to
use 96-well plates.
What is very
practical is the fact that the thermal blocks operate independently,
not only in terms of PCR protocols, but also because they can still
operate even if one or more of the blocks is out of order.
In summary,
the DNA Engine Tetrad is one of the very few high-throughput PCR
machines available on the market and I think it is a good one. It’s
compact design and modular structure facilitate performing large
scale experiments. I recommend this device to any researcher
planning to start large-scale genomics projects.
Feature Overview
Swappable Alpha unit sample
blocks are designed for frequent change; they can be swapped in
seconds; they seat many types of reaction vessels.
Independently controlled dual
blocks are available for running two different protocols
simultaneously.
Unique Peltier heat pumps
are built by MJ Research specifically for cycling. Years of
experience and proprietary technologies make MJ modules last longer
than any other.
Multi zone
feedback control utilizes multiple sensors combined with
four “zones” for heating and/or cooling to optimize thermal profiles
every 50 milliseconds.
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