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The Thermo Luminoskan Ascent
is a sensitive and flexible microplate luminometer. Combined with the
powerful
Ascent Software,
it handles both glow-and flash-type reactions. The instrument covers a
wide range of research, industrial and clinical applications.
Luminoskan Ascent offers great advantages for many
different types of assays. These include ATP-based assays for hygiene
and sterility monitoring, or quality control in the food, pharmaceutical
and healthcare industries. Reporter gene assays, such as luciferase and
galactosidase, are widely performed in research and routine molecular
biology laboratories. Luminescent immunoassays and DNA hybridization
assays based on luminescent enzyme substrates or luminescent labels are
other important application areas for this instrument.
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Application areas of the Luminoskan Ascent |
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Reporter gene assays |
ATP assays |
Cytotoxicity |
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Immunoassays |
DNA quantitation |
Cell proliferation |
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Phagocytosis |
Intracellular Ca2+ |
Enzyme assays |
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Microbiological assays |
Reactive oxygen assays |
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Completely robot compatible
Robotic integration is simple, effective and easy to
automate with the Luminoskan Ascent.
The
plate carrier allows convenient access for different kinds of robotic
arms. It has been designed to enable portrait gripping of the plate with
the gripper of less than 60 mm and also landscape gripping with the
gripper of less than 65 mm. In addition, wall edges of the carrier have
been beveled to aid the plate to slide
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High-performance optics
Luminoskan incorporates the latest optical design. High sensitivity is
one of the main benefits of the fiberless optics and a critical feature
for luminometric measurements. It measures less than 1 fmol ATP per well
and has a wide dynamic range of more than 9 decades for maximum
flexibility. Luminoskan Ascent can also be equipped with filters. The
luminometer reads microplate formats from 1-96 and 384-well plates.
Versatility through dispenser system, incubator and shaker
For an
easy and accurate addition of reagents, up to three dispensers can be
fitted on-board the Luminoskan Ascent. The dispensers allow a precise
delivery of reagents in 1 ”l increments over an adjustable volume range
of 1-1000”l. Speed and precision are important advantages of these
dispensers.
For
assays requiring precise temperature control, Luminoskan Ascent features
an on-board incubator. Built-in orbital shaking, another advanced
feature, ensures effective mixing and speeds up reactions. Both the
speed and amplitude of the shaking can be adjusted. For flash
luminescence reactions performed with acridinium esters and aequorin,
for example, Ascent Software supports simultaneous reagent injection and
signal monitoring. The ability to add reagents and read in any sequence
allows multiphase ATP and reporter genes assays.
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