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MegaBACE 1000 DNA Sequencer
The
MegaBACE 1000 is an automated machine capable of high-throughput DNA
analysis, processing 96 samples in just a few short hours.
Applications that are compatible with this system are DNA
sequencing, genotyping, and fragment analysis.
The
MegaBACE 1000 has 6 arrays of 16 capillaries with an interior
diameter of about 100 µm. The system uses high-pressure nitrogen gas
to inject the capillaries with Linear Polyacrylamide, a denaturing
gel. To load samples, a 96-well plate is loaded into the machine and
a current is set up at the tip of each capillary where it comes into
contact with the sample. DNA (and other charged particles in the
sample) are electro-kinetically injected into the Linear
Polyacrylamide and travel the length of the capillary during the
course of the run. A typical sequencing run takes about 4 hours
while genotyping and fragment analysis takes only a little over 2
hours. All sequencing samples are automatically base-called. After
the run, data from all samples are ready to be exported to other
types of DNA analysis programs.
Because of the electro-kinetic injection, initial optimization for a
specific set of samples is critical. The ratio of labeled DNA to
unlabeled DNA and other charged particles plays a large role in the
success of any type of analysis. After optimization, the 96-well
capacity of the MegaBACE allows the processing of large numbers of
samples in a relatively short amount of time.
You can reserve/order this instrument
by calling 763-712-8717 or by
completing the form on the
Ordering Page.
As this instrument is outside the scope of GMI service repertoire
we will take any reasonable offer. The instrument was
deinstalled from a hospital after not being used for a while.
It comes with a computer and software. What we got, you get.
Make an offer today!
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