Complete Product Description

Vi-Cell automated Cell Viability Analysis

The Beckman Coulter Vi-Cell Automated Cell Viability Analyzer automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Beckman Coulter Vi-Cell Automated Cell Viability Analyzer automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

For confirming viable cell populations. Therefore, both cell concentration and percentage of viable cells must be determined accurately and precisely. The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Beckman Coulter Vi-Cell Automated Cell Viability Analyzer is simple to operate

A cell sample is loaded into the system (minimum 600uL, but can be diluted and dilution factored into final result), and the instrument aspirates the sample, mixes in the trypan blue, loads the sample into the chamber and records: cell count/mL (equivalent of 100 ?grids?), percent viability, and size distribution. It then performs a clean cycle and repeats for the next sample in the carousel. Sample processing time is approximately 2.5 minutes per sample, although there are several user programmable features that can reduce or increase this time.

Real life application of the Beckman Coulter Vi-Cell Automated Cell Viability Analyzer

At the end of the 19th Century, Pasteur?s research showed that alcohol production from glucose was linked directly to yeast. The fermentation property of yeast is utilized in the beer, wine, and ethanol production industries. For optimum yeast fermentation performance, percentage cell viability and concentration must be monitored. The Beckman Coulter Vi-Cell Automated Cell Viability Analyzer automates the labor intensive manual trypan blue viability method. In addition, the Vi-CELL reports accurate and precise cell concentrations. The Vi-CELL provides the high resolution necessary for accurate results on yeast and other small cells.