The FACS Canto Flow Cytometer has two lasers an air cooled 20mW solid state blue laser which excites at 488nm.

The other is a 17mW HeNe red laser which excites at 633nm which allows for 6-colour analysis.

Currently the FACS Canto Flow Cytometer can detect the following blue laser fluorescent dyes:

• FITC (525nm)
• PE (575nm)
• PerCP (678nm) or PerCP-Cy5.5 (695nm) and PE-Cy7(785nm).

The red laser dyes the FACS Canto Flow Cytometer can detect are APC (660nm) and APC-Cy7 (785nm). Together with 2 light scatter detectors this cytometer is the latest cutting edge technology that makes 8-parameter analysis a breeze.

• Cellular metabolism studies: including changes in intracellular calcium levels cellular pH and changes in membrane potential
• DNA analysis/ploidy/karyotyping: Studies of changes in DNA/RNA levels using DNA-binding dyes such as Ethidium bromide.
• Cytotoxicity studies: including studies of Natural killer cell activity and Cytotoxic T lymphocyte (CTL) activity.