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BD FACS Calibur Flow Cytometer

BD FACS Calibur Flow Cytometer

  • Dual Lasers capability Optimized for Multicolor Analysis
  • Automated sample loading option
  • Powerful software for high throughput
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Complete Product Description

The BD FACS Calibur Flow Cytometer system is another BD first: the only four-color, dual-laser, benchtop system available today that is capable of both cell analysis and sorting. Designed specifically to support a wide range of applications, the BD FACSCalibur system is a fully integrated multiparameter system.

The BD FACS Calibur Flow Cytometer combines unique dual-laser technology, an automated sample loader option, and powerful software to provide the high throughput necessary to meet productivity requirements of clinical laboratories. The modularity and innovative technology designed into the BD FACSCalibur system also offers investigators the performance and flexibility required for a variety of research applications.

The built-in versatility of the BD FACS Calibur Flow Cytometer meets the current demands of both clinical and research environments. Its flexibility will help you meet tomorrows demands

The BD FACSCalibur system can quickly perform routine tasks including immunophenotyping, absolute counting, residual white blood cell enumeration, stem cell analysis, DNA analysis, and isolation by sorting. And with its superior multicolor analysis and sorting capabilities, the BD FACSCalibur system is a highly sensitive tool, providing laboratories with the technological capabilities crucial for sophisticated cellular and molecular work in areas such as genetics, proteomics, multicolor analysis, and immune function.

Dual Lasers capability Optimized for Multicolor Analysis

The BD FACSCalibur system offers the industry standard in multicolor analysis capability?two lasers spatially separated for high sensitivity, minimal compensation, and maximum flexibility in fluorochrome selection. With multicolor analysis, you maximize the information gained from small samples, as well as increase the cost-effectivness of each assay.

BD FACSCalibur Flow Cytometer System Specifications

Laser
488-nm, air-cooled Argon-ion laser
635-nm, air-cooled red diode laser (optional)
Fluorescence Sensitivity
Estimated detection limit is 200 FITC equivalent molecules per particle.
Fluorescence Resolution
Coefficient of variation of <3%, full peak, for propidium iodide?stained chicken erythrocyte nuclei
Forward and Side Scatter Sensitivity
Sensitivity enables the separation of fixed platelets from noise.
Forward and Side Scatter Resolution
Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.
Sorting Specification
Purity >95%
Rate Up to 300 particles per second
Modes Single Cell, Exclusion, and Recovery

BD FACSCalibur Flow Cytometer Optional Accessories

  • FL4 Option
    • Four-color fluorescence capability
    • Red diode second laser
      • High sensitivity
      • Minimal compensation
  • Sorting Option
    • Minimzes sort setup
    • Enhances safety with aerosol-free sorting
  • Cell Concentrator Module
    • Sorts cells directly onto filters or cell culture inserts
  • BD FACS Loader
    • Automates sample introduction for 12 x 75-mm tubes

Includes the BD FACStation Data Management System

The ease-of-use of the BD FA C S C a l i b u r system is enhanced by its user- friendly data management system. The BD FACStation workstation takes advantage of the intuitive Macintosh environment by using simple pull-down menus and icons. It performs all the computing tasks required for fast, accurate results, including instrument setup as well as data collection, analysis, and management. The BD FACStation workstation offers automated software and high-performance acquisition and analysis tools for plotting, gating, statistical analysis, and reporting.